iMatrix-511/-511 silk Pre-mix method (WITHOUT PRE-COATING)

【 Download 】Protocol, CATALOG  

Advantages of using iMatrix-511/-511 silk

 
  • No pre-coating required
  • Requires half iMatrix-511 /-511 silk
  • No loss of cells and culture dishes

●　No pre-coating required

●　Requires half iMatrix-511 /-511 silk

The pre-mixed method markedly reduced the amount of substrate needed for the maximum adhesion of hPSCs compared with the pre-coat method.
You can obtain the same results using half iMatrix-511/-511 silk.

●　No loss of cells and culture dishes

Have you had difficulties with cell passages? – The number of cells are not enough to the number of dishes. – The number of cells are too many for the number of dishes.
【 Reference 】 Miyazaki et al. Efficient adhesion culture of human pluripotent stem cells using laminin fragments in an uncoated manner. Scientific Reports, 7, 41165, (2017). 【 Press release 】 Below above Kyoto University WEB site. URL:http://www.kyoto-u.ac.jp/ja/research/research_results/2016/170130_1.html